We propose Master projects (min. 6 months) on these two themes:
1. To measure promoter transcription as a function of enhancer location and their mutual interaction probabilities, we recently developed a new assay enabling an enhancer to be positioned at large numbers of chromosomal sites around its target promoter (see our recent preprint). We would like a student to contribute to the development of a new version of this assay allowing to measure many enhancers and promoters in parallel.
2. Understanding enhancer-promoter communication mechanistically ultimately requires to measure how they interact in single living cells. To this end we use live-cell imaging techniques allowing us to track the position of an enhancer and a promoter in real time within the nucleus, as well as the RNA that is produced by the promoter. We are looking for a student to help with cell line engineering, microscopy and data analysis.
The student(s) working on these projects will:
- Use CRISPR/Cas9 and transposons to engineer mouse embryonic stem cells
- Perform live-cell imaging on state-of-the-art microscope systems
- Learn basic methods of quantitative imaging data analysis.
We are looking for a highly motivated and self-driven student with knowledge of basic molecular biology techniques. Experience with cell culture, microscopy and image processing is an advantage. Candidates should apply by email with a CV listing courses and internships taken during Masters studies.